Ocorrência do gene mecA em Staphylococcus spp isolados da pele de cães com piodermite recidivante.

Abstract

Pyoderma can be defined as an infectious condition producing pus with a bacterial cause that affects the skin. Staphylococcus spp is the bacterium most frequently involved in these infections in dogs. Canine atopic dermatitis (CAD) is an inflammatory and pruriginous skin disease of dogs with clinical characteristics and related to a type I hypersensitivity response, targeted to immunoglobulin E (IgE) antibodies mainly against environmental allergens. CAD has a genetic component and leads to loss in the protective barrier of the skin, causing their carriers to present cases of recurrent pyoderma. We selected 25 dogs of various races, age and no sexual preference. All were clinically diagnosed as having recurrent pyoderma and CAD. Purulent material was collected for culture in Blood Agar and Mackonkey Agar and by means of microbiological characteristics to isolate Staphylococcus spp and subsequent PCR and qPCR for detection of the mecA gene, and finally confirming with the nucleotide sequencing of the positive samples. Nine (36%) samples were positive in one or both PCR techniques. As a first research on the topic performed in the region and with the result quite representative, we concluded that the detection of the gene in these microorganisms isolated from dogs with very common dermatological diseases, make us reflect on a better therapeutic approach, aiming at a better quality of health human and animal.