Detecção molecular de Bartonella spp. no tecido hepático de quirópteros neotropicais.

Abstract

The genus Bartonella is composed of gram-negative bacilli that are used by tropism by erythrocytes and endothelial cells, Rodentia, Lagomorpha, Carnivora, Artiodactyla, Eulipotyphla and Chiroptera. Bacterial infection may be associated with lymphadenitis, endocarditis, bacillary angiomatosis and hepatic peliosis. Thirteen species of Bartonella are considered as zoonotic and, the chiroptera, as potential reservoirs of the agent. In this sense, it was aimed to detect the occurrence of Bartonela spp. in livers of neotropical bats. A total of 316 livers from chyloptera livers belonging to the families Molossidae, Phyllostomidae and Vespertilionidae were analyzed in 21 genera from the Atlantic forest biome of the State of São Paulo for partial amplification of the gltA gene of the genus Bartonella spp. by polymerase chain reaction (PCR). Samples obtained from two bats (0.6%) of the species Glossophaga soricina from the municipality of Franca as positive by PCR. The amplicons were sequenced and as consensus sequences were aligned with representative nucleotide sequences of the genus Bartonella for phylogenetic analysis. A phylogenetic analysis indicated that as sequences clustered in a clade near the sequence of Bartonella sp. detected in bat of the species Glossophaga soricina collected without Cerrado biome, in the State of Tocantins, Brazil.