Níveis de expressões dos genes defensinas (CBD1 E CBD103) em raspados cutâneos de cães da raça poodle com e sem dermatite.

Abstract

Defensins are an important group of antimicrobial peptides and are expressed on the skin and other tissues of various mammalian species. These peptides participate in innate immunity, aiding in the immune response. The aim of the study was to compare the levels of β-defensin gene expression (CBD1 and CBD103) in skin scrapings of Poodle dogs with and without dermatitis. Eight dogs with dermatitis and 13 healthy dogs from the Veterinary Hospital and privately owned were used. Samples were collected by skin scraping and RNA extraction obtained by Trizol® reagent. mRNA expression levels were estimated by RT-PCR using the SYBR® Green One-step Kit on the CFX96 Touch ™ Real-Time PCR Detection System (BioRad, Hercules, CA, USA). Data were compared by 5% Student's t-test. Relative expressions of the CBD1 and CBD103 genes were significantly higher in dogs with dermatitis. Dogs with dermatitis showed higher levels of defensin expression, suggesting a possible immune response in the skin of these animals.