Avaliação da oxidação de segmentos de ráquilas de açaizeiro (Euterpe Oleracea, Mart.) sob diferentes condições de cultura in vitro.

Abstract

The objectives of this work were to evaluate the efficiency of different antioxidants in the culture medium and the effect of the stage of development of açai inflorescence (Euterpe oleracea Mart.) on the intensity of oxidation of explant segments cultivated in vitro. The experimental activities were held in the Laboratory of Genetic Resources and Biotechnology of Embrapa Oriental, Amazon Basin, Belém (PA). The rachis segments, obtained from açai aseptic inflorescence, were previously inoculated in tubes of solid and liquid MS medium (Murashige & Skoog, 1962) with 3% of sucrose and supplemented with different antioxidants. The cultures were kept in growth room under total absence of light. After 15 days of inoculation, the oxidation intensity and the number of contaminated explants were determined. The young rachis segments showed little oxidation intensity. The mature rachis segments only reached small oxidation intensity when inoculated in a solid MS medium with 0,3% of activated charcoal, in the presence or absence of ascorbic acid. The other antioxidants when associated to the liquid medium did not show a satisfactory control of the oxidation intensity on young and mature açai rachis segments.