https://repositorio.ufra.edu.br/jspui/handle/123456789/1394 2026-04-09T19:11:26Z https://repositorio.ufra.edu.br/jspui/handle/123456789/2139 Título: Effect of age and sex in renal function by ultrasound and serum chemistry in two primate species (Alouatta caraya and Sapajus apella). Autor(es): SILVA, Gessiane Pereira da; PEREIRA, Thyago Habner de Souza; IMBELONI, Aline Amaral; ANDRADE, Rafael do Santos de; DIAS NETO, Ramiro das Neves Dias; COUTINHO, Leandro Nassar; GERING, Ana Paula; BENARRÓS, Marina Sette Camara; PEREIRA JÚNIOR, José Jones; MONTEIRO, Frederico Ozanan Barros Monteiro; TAKESHITA, Rafaela Sayuri Cicalise Abstract: Estudos comparativos da morfofisiologia renal em primatas não humanos podem nos ajudar a investigar diferenças interespécies nos padrões de crescimento e envelhecimento. Métodos: Testamos o efeito da idade e do sexo na morfofisiologia renal em 21 Alouatta caraya e 21 Sapajus apella (faixa etária = 0,5–26 anos) por ultrassonografia, contagem de glóbulos vermelhos (RBC) e testes de função renal. 2023-12-22T00:00:00Z https://repositorio.ufra.edu.br/jspui/handle/123456789/2137 Título: Validation of a dehydroepiandrosterone-sulfate assay in three platyrrhine primates (Alouatta caraya, Aotus azarae infulatus and Sapajus apella). Autor(es): SILVA, Gessiane Pereira da; MELO, Josi Teixeira de; MONTEIRO, Frederico Ozanan Barros; FERREIRA, Ana Karolyna Pereira; CARNEIRO, Liliane Almeida; TAKESHITA, Rafaela Sayuri Cicalise Abstract: The hormone dehydroepiandrosterone (DHEA) and its sulfate ester (DHEAS) are the most abundant circulating steroids in human and some nonhuman primates, and have been implicated in development, aging and in stress modulation. We aimed to validate a commercially available enzyme immunoassay kit to measure DHEAS in feces and serum of three platyrrhine primate species (Alouatta caraya, Aotus azarae infulatus, and Sapajus apella) in captivity. We collected serum samples from one male and one female from each species. To validate the kit for fecal samples, we conducted a physiological validation by administering DHEA orally to one adult female of each species. We also measured fecal DHEAS levels in four female Alouatta caraya individuals during the third semester of gestation and in two females following parturition. We obtained a total of 54 fecal samples and 6 serum samples from 10 individuals. We validated the assay analytically by testing parallelism and accuracy tests in both fecal and serum extracts for all species. We observed a peak in DHEAS 24 h following oral DHEA administration in all three species, with A. caraya presenting the strongest response and highest baseline concentrations. DHEAS levels were elevated in pregnant A. caraya (57,843.86 ± 37,160.31 ng/g) and declined after parturition (1,539.07 ± 2,894.74 ng/g). Our results demonstrated that these platyrrhines secrete measurable concentrations of DHEAS, with A. caraya secreting levels comparable to those of catarrhines. The EIA kit is valid for quantification of fecal and serum DHEAS, and it is useful for studies on stress and primate evolution. 2023-12-15T00:00:00Z https://repositorio.ufra.edu.br/jspui/handle/123456789/2136 Título: Hematological and serum biochemistry evaluation in howler monkeys (Alouatta caraya) and capuchin monkeys (Sapajus apella): A comparative study. Autor(es): SILVA, Gessiane Pereira da; PEREIRA, Thyago Habner de Souza; MELO, Josi Teixeira de; IMBELONI, Aline Amaral; ANDRADE, Rafael dos Santos de; MONTEIRO, Maria Vivina Barros; MONTEIRO, Frederico Ozanan Barros; TAKESHITA, Rafaela Sayuri Cicalise Abstract: Background: Evaluation of blood parameters in captive non-human primates (NHPs) is crucial for monitoring their health and ensuring that their environment meets their physiological requirements. Methods: We performed hemogram, serum biochemistry, and parasitological exams in 20 howler monkeys and 21 capuchin monkeys. Results: In both species, over 50% of the individuals presented at least one parasite. There was a negative effect of age on red blood cell (RBC), white blood cell, platelets, total protein, globulin, and alkaline phosphatase, and a positive effect on the A:G ratio, gamma-glutamyl transferase, and mean platelet volume (MPV). Capuchin monkeys presented the highest platelets and alanine aminotransferase (ALT) values and howler monkeys presented the highest MPV, aspartate aminotransferase, ALT, amylase, glucose, bilirubin, and triglycerides values. We observed an interaction between species and sex on RBC, Htc, mean corpuscular hemoglobin concentration, and cholesterol. Conclusions: Species differences found in blood parameters may reflect differences in physiological adaptations associated with ecological and morphological traits and are clinically relevant for evaluating animal health and the suitability of breeding programs. 2023-12-13T00:00:00Z https://repositorio.ufra.edu.br/jspui/handle/123456789/2133 Título: Female squirrel monkeys as models for research on women’s pelvic floor disorders Autor(es): SILVA, Gessiane Pereira da; PEREIRA, Thyago Habner de Souza; LIMA, Ana Kelen Felipe; VICENTE, Wilter Ricardo Russiano; KUEHL, Thomas J.; RUIZ, Julio Cesar; MONTEIRO, Frederico Ozanan Barros Abstract: Animal models enable research on biological phenomena with controlled interventions not possible or ethical in patients. Among species used as experimental models, squirrel monkeys (Saimiri genus) are phylogenet- ically related to humans and are relatively easily managed in captivity. Quadrupedal locomotion of squirrel monkeys resembles most other quadrupedal primates in that they utilize a diagonal sequence/diagonal couplets gait when walking on small branches. However, to assume a bipedal locomotion, the human pelvis has undergone evolutionary changes. Therefore, the pelvic bone morphology is not that similar between the female squirrel monkey and woman, but pelvic floor support structures and impacts of fetal size and malpresentation are similar. Thus, this review explores the pelvic floor support structural characteristics of female squirrel monkeys, especially in relation to childbirth to demonstrate similarities to humans. 2023-12-12T00:00:00Z