DSpace Communidade: Programa de Pós-Graduação em Biotecnologia Aplicada à Agropecuária

Condições hídricas e térmicas para o desenvolvimento ex vitro e in vitro de Acmella oleracea (L.) R.K. Jansen (Jambu).

2026-05-25T21:35:40Z

Título: Condições hídricas e térmicas para o desenvolvimento ex vitro e in vitro de Acmella oleracea (L.) R.K. Jansen (Jambu). Autor(es): BASTOS, Ana Lúcia Santiago de Oliveira Abstract: Jambu, Acmella oleracea (L.) R.K. Jansen, is a native vegetable from the Amazon with high socioeconomic value for small producers in Pará and other regions of Brazil. In this context, the generation of strategic information is essential to support future genetic breeding programs aimed at developing cultivars adapted to environments with greater climatic adversity. The present study aimed to evaluate the response to thermal and water stress in two jambu varieties (“yellow” and “purple”) under in vitro and ex vitro conditions to guide management strategies and improve the production system. In the water stress experiment, five irrigation levels (40, 70, 100, 130, and 160% of field capacity) were applied in a nursery, evaluating morphological variables (shoot length, root length, number of leaves, number of branches, number of nodes, number of roots, bud opening, and inflorescences) and biomass of leaves, roots, inflorescences, and shoot length. In the thermal stress assay, germination and initial seedling development morphology in vitro were analyzed under temperatures of 25, 33, and 41 °C. A completely randomized design (CRD) was used in both experiments. It was evidenced that water availability and temperature distinctly modulate the phenotypic expression of plants. The purple variety demonstrated high phenotypic plasticity under ideal irrigation conditions (100% and 130% of field capacity). PCA confirmed this trend, associating the variety with greater reproductive investment and leaf area expansion. The yellow variety adopted a conservative morphological strategy, prioritizing physiological stability. Severe water deficit restricted vegetative growth in both varieties and predisposed plants to mite attacks. In in vitro cultivation, 25 °C proved ideal for germination and seedling development, while 33 °C induced significant stress, with etiolation, reduced leaf area, and compromised rhizogenesis. The temperature of 41 °C completely prevented germination. It is concluded that jambu cultivation is highly dependent on proper management of water and thermal conditions, with recommended irrigation levels close to 100–130% of field capacity and 25 °C in in vitro cultivation. In the face of extreme climatic events, the purple variety stood out as the most suitable for intensive cultivation systems due to its greater phenotypic plasticity and productive stability in the face of environmental variations.

Bioprospecção do genótipo de resistência em solo e rizosfera de Piper nigrum e a influência do tutor na seleção desses genes

2026-05-12T19:43:23Z

Título: Bioprospecção do genótipo de resistência em solo e rizosfera de Piper nigrum e a influência do tutor na seleção desses genes Autor(es): CHAVES, Valéria Alves Abstract: Brazil is the second largest global exporter of black pepper (Piper nigrum), with a production of approximately 126,000 tons in 2023, intended for both export and domestic consumption. However, production faces significant challenges, particularly those related to diseases caused by phytopathogens, which directly impact productivity. The cultivation of this species can be carried out using wooden stakes or live tutors, the latter being preferred due to lower costs, reduced deforestation associated with wood extraction, and a lower demand for fertilizers. Due to the intensified use of agricultural inputs in export-oriented cropping systems, it becomes relevant to understand the natural defense mechanisms of plants, such as the recruitment of beneficial microorganisms by the rhizosphere. Among these, Actinobacteria stand out for their ability to produce antimicrobial compounds and to act in the biocontrol of phytopathogens. Thus, this study aims to evaluate the influence of different tutoring systems (live and dead) on the diversity and composition of the rhizosphere microbiota of Piper nigrum, with an emphasis on the recruitment of actinobacteria and the characterization of antimicrobial resistance genes present in the samples.

Vitrificação em fluido folicular bovino.

2026-03-17T18:14:03Z

Título: Vitrificação em fluido folicular bovino. Autor(es): MERCÊS, Marcela Oliveira das Abstract: Vitrification is one of the methods used for immature and mature bovine oocytes. However, it needs improvement regarding hearing competence after warming. The use of bovine follicular fluid (BFF) is known in in vitro production systems, but unexplored in vitrification media. The aim of this study was to vitrify oocytes in a solution supplemented with bovine follicular fluid (BFF) or fetal bovine serum (FBS) to investigate post-warming survival through nuclear maturation and analysis of HSP70 gene expression. The study was divided into two experiments: In experiment 1, immature cumulus oophorus complexes (COCs) were distributed into three experimental groups. In experiment 2, three experimental groups were used for mature oocytes and three experimental groups for immature cumulus oophorus complexes. The control group of oocytes (without vitrification) in immature oocytes were directly subjected to in vitro maturation. Both experiments used, in group 1, (TCM 199 medium + 20% fetal bovine serum (FBS) + cryoprotectants), and in group 2, vitrification in (TCM 199 medium + 20% bovine follicular fluid (BFF) + cryoprotectants), followed by in vitro maturation of immature oocyte complexes (OCCs). In experiment 1, all immature oocytes from the experimental groups, after maturation, were stained with acetic orcein for structural visualization. Meanwhile, in experiment 2, matured and immature oocytes, after warming, were subjected to gene expression analysis of the heat shock protein HSP70. Survival rates of oocytes that were matured to assess meiotic stage II (MII) were expressed as percentages and evaluated by analysis of variance (ANOVA) and Bonferroni post-hoc test. For statistical analysis, SigmaPlot® 14.5 software was used with a significance level of P<0.005. The statistical analysis of gene expression was performed using two-way ANOVA in the Sigmaplot program with a significance level of 0.05. In the maturation analysis of immature oocytes, no significant differences were found between the groups vitrified with FBS or FFB (p>0.001), and the maturation rate was lower (p>0.001) than the control group. Regarding experiment 2, oocytes from the vitrified matured group, the gene expression analysis of the HP70 protein showed no difference between the groups vitrified with FBS and FFB, while the granulosa cells of immature oocytes, FFB, and FBS also differed from each other and from the control (p < 0.05). These results indicate that follicular fluid can be used to replace FBS supplementation in vitrification media of bovine oocytes.

Montagem, predição gênica e anotação do genoma da Cynoscion acoupa (Lacepède, 1801).

2025-07-14T11:59:57Z

Título: Montagem, predição gênica e anotação do genoma da Cynoscion acoupa (Lacepède, 1801). Autor(es): OLIVEIRA, Rosyely da Silva Abstract: Aquaculture in Brazil had positive results in 2023, where national production reached 887,03 tons. The Cynoscion acoupa is a fish popularly known as “pescada-amarela”, found mainly on the north coast and has a great economic value mainly for gastronomic purposes. More recently, a material called "isinglass" was observed, found in the swim bladder of this fish, which has been used as raw material in several areas of the market, such as the pharmaceutical, civil, food, among others. The purpose of this work is to perform the genome assembling, gene prediction and carry out functional annotation of the C. acoupa organism. DNA samples were extracted from 5 specimens in the regions of Salinópolis-PA, Bragança-PA and on the coast of Amapá. The best sample was chosen to continue the study. Through these samples, the complete genome of the captured individuals was sequenced, with two paired libraries paired-end short insert (2 x 250 bp) DNAseq built with the Illumina DNA prep Kit, using the NovaSeq SP 6000 Illumina sequencing platform, producing raw data. Subsequently, FASTQC were used to verify the quality of the sequencing. From the generated sequences the de novo genome assembly will be made using the ABYSS assembler. BOWTIE-2 will be used to align the contigs and scaffolds with the genome of a phylogenetically close species, GeneMark will be used for gene prediction and BUSCO for genome annotation, so that it can finally be deposited at the NCBI. Through the results, it is expected to bring new information about genome sequencing and its quality, together with the publication of these data for future studies.